TY - JOUR T1 - Catecholaminergic cell lines from the brain and adrenal glands of tyrosine hydroxylase-SV40 T antigen transgenic mice JF - The Journal of Neuroscience JO - J. Neurosci. SP - 1280 LP - 1291 DO - 10.1523/JNEUROSCI.13-03-01280.1993 VL - 13 IS - 3 AU - C Suri AU - BP Fung AU - AS Tischler AU - DM Chikaraishi Y1 - 1993/03/01 UR - http://www.jneurosci.org/content/13/3/1280.abstract N2 - Brain (CATH.a) and adrenal (PATH.1 and PATH.2) cell lines have been established that synthesize abundant dopamine and norepinephrine and express the appropriate catecholaminergic biosynthetic enzymes, tyrosine hydroxylase (TH) and dopamine beta-hydroxylase. The lines were derived from TH-positive tumors in transgenic mice carrying the SV40 T antigen oncogene under the transcriptional control of 773 base pairs of 5′ flanking sequences from the rat TH gene. Although the lines continue to express T antigen, they exhibit neuronal properties such as neurofilaments and synaptophysin and lack glial intermediate filaments. Although in vivo TH is only expressed in postmitotic neurons in the CNS, the CATH.a line demonstrates that TH expression and continued cell division are not incompatible after oncogenic transformation. ER -