RT Journal Article
SR Electronic
T1 Interleukin-1 beta augments release of norepinephrine, dopamine, and serotonin in the rat anterior hypothalamus
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 3574
OP 3581
DO 10.1523/JNEUROSCI.13-08-03574.1993
VO 13
IS 8
A1 Shintani, F
A1 Kanba, S
A1 Nakaki, T
A1 Nibuya, M
A1 Kinoshita, N
A1 Suzuki, E
A1 Yagi, G
A1 Kato, R
A1 Asai, M
YR 1993
UL http://www.jneurosci.org/content/13/8/3574.abstract
AB We investigated the effects of interleukin-1 beta (IL-1 beta), administered directly into the rat anterior hypothalamus (AHY), on monoamine release in the same region by using a brain microdialysis technique and an HPLC-electrochemical detection system. First, to study the local effects of IL-1 beta, we used a microdialysis probe equipped with a microinjection tube for administering IL-1 beta in the same region into which the probe had been inserted. IL-1 beta (1 ng) injected directly into the AHY elicited release of norepinephrine (NE), dopamine (DA), and 5-HT, as well as increases in their metabolites, 4- hydroxy-3-methoxyphenylglycol, 3,4-dihydroxyphenylacetic acid, 4- hydroxy-3-methoxyphenylacetic acid, and 5-hydroxyindole-3-acetic acid, in the AHY. Vehicle alone exerted no effect on monoamine release. Although the elevated levels of NE and DA persisted for more than 6 hr after injection of IL-1 beta, the elevated levels of 5-HT were transient. Second, in order to investigate whether this effect of IL-1 beta is a direct action in the AHY, we performed in vitro experiments using hypothalamus slices. IL-1 beta (0.1 and 1 nM) increased the levels of each monoamine released from hypothalamic slices in a dose- dependent manner. These findings suggest that IL-1 beta acts directly on the hypothalamus to induce release of NE, DA, and 5-HT. Third, the roles of prostaglandins (PGs) in NE release in the AHY elicited by direct injection of IL-1 beta were examined.(ABSTRACT TRUNCATED AT 250 WORDS)