RT Journal Article
SR Electronic
T1 Observation and quantitation of exocytosis from the cell body of a fully developed neuron in Planorbis corneus
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 7747
OP 7755
DO 10.1523/JNEUROSCI.15-11-07747.1995
VO 15
IS 11
A1 Chen, G
A1 Gavin, PF
A1 Luo, G
A1 Ewing, AG
YR 1995
UL http://www.jneurosci.org/content/15/11/7747.abstract
AB We have discovered a neuronal system that releases neurotransmitter via exocytosis from the cell body. In the large dopamine cell of the pond snail Planorbis corneus, depolarization induces rhythmic release of dopamine from the cell body. When a stimulant is applied extracellularly or intracellularly in situ to the cell body, transient dopamine concentration packets that appear in a bursting pattern are observed. Dopamine release is calcium dependent and release is on the time scale expected for exocytosis (2 to 4 msec rise times). Quantitation of individual events reveals an average of 818,000 molecules per exocytotic event. As many as 89,000 individual exocytotic events have been observed following a single stimulation of one cell. Neurotransmitter exocytosis from the neuronal cell body appears to represent an alternative form of neurocommunication to synaptic transmission.