RT Journal Article
SR Electronic
T1 Nerve injury enhances rat neuronal glutamate transporter expression: identification by differential display PCR
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 7872
OP 7878
DO 10.1523/JNEUROSCI.15-12-07872.1995
VO 15
IS 12
A1 S Kiryu
A1 GL Yao
A1 N Morita
A1 H Kato
A1 H Kiyama
YR 1995
UL http://www.jneurosci.org/content/15/12/7872.abstract
AB An increase in neuronal glutamate transporter expression after nerve injury was demonstrated by means of differential display PCR (DD-PCR) coupled with in situ hybridization. DD-PCR was carried out to compare differences in expression of mRNAs between axotomized and normal hypoglossal motoneurons in the rat. The expression of several gene fragments were found to be increased following nerve injury; the full length cDNA corresponding to one fragment was cloned by subsequent rat cDNA library screening. The close homology of glutamate transporters with our rat cDNA led us to conclude that this clone corresponds to the rat neuronal glutamate transporter (rat EAAC1). We speculate that the upregulation of this glutamate uptake system may increase the resistance of these cells against neurotoxic glutamate accumulation during the process of nerve regeneration.