PT  - JOURNAL ARTICLE
AU  - D Sulzer
AU  - TK Chen
AU  - YY Lau
AU  - H Kristensen
AU  - S Rayport
AU  - A Ewing
TI  - Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport
AID  - 10.1523/JNEUROSCI.15-05-04102.1995
DP  - 1995 May 01
TA  - The Journal of Neuroscience
PG  - 4102--4108
VI  - 15
IP  - 5
4099  - http://www.jneurosci.org/content/15/5/4102.short
4100  - http://www.jneurosci.org/content/15/5/4102.full
SO  - J. Neurosci.1995 May 01; 15
AB  - Whether amphetamine acts principally at the plasma membrane or at synaptic vesicles is controversial. We find that d-amphetamine injection into the Planorbis giant dopamine neuron causes robust dopamine release, demonstrating that specific amphetamine uptake is not required. Arguing for action at vesicles, whole-cell capillary electrophoresis of single Planorbis dopamine neurons shows that amphetamine reduces vesicular dopamine, while amphetamine reduces quantal dopamine release from PC12 cells by > 50% per vesicle. Intracellular injection of dopamine into the Planorbis dopamine neuron produces rapid nomifensine-sensitive release, showing that an increased substrate concentration gradient is sufficient to induce release. These experiments indicate that amphetamine acts at the vesicular level where it redistributes dopamine to the cytosol, promoting reverse transport, and dopamine release.