PT  - JOURNAL ARTICLE
AU  - WE Lyons
AU  - JP Steiner
AU  - SH Snyder
AU  - TM Dawson
TI  - Neuronal regeneration enhances the expression of the immunophilin FKBP- 12
AID  - 10.1523/JNEUROSCI.15-04-02985.1995
DP  - 1995 Apr 01
TA  - The Journal of Neuroscience
PG  - 2985--2994
VI  - 15
IP  - 4
4099  - http://www.jneurosci.org/content/15/4/2985.short
4100  - http://www.jneurosci.org/content/15/4/2985.full
SO  - J. Neurosci.1995 Apr 01; 15
AB  - Immunophilins are a group of proteins that serve as receptors for the immunosuppressant drugs cyclosporin A and FK506. The immunophilin designated FK-506 binding protein-12 (FKBP-12) is concentrated more than 10 times higher in the brain than in immune tissues. The complex of FK506 and FKBP-12 inhibits the calcium activated phosphatase, calcineurin, increasing phosphorylated levels of calcineurin substrates with growth associated protein-43 (GAP-43), being most prominent in the brain. We now demonstrate an association of FKBP-12 with neuronal regeneration and GAP-43 disposition. Facial nerve crush markedly augments expression of FKBP-12 mRNA in the facial nucleus with a time course paralleling changes in GAP-43 mRNA. Following sciatic nerve lesions, similar increases in FKBP-12 mRNA occur in lumbar motor neurons and dorsal root ganglia neuronal cells. Increased FKBP-12 expression appears linked to regeneration rather than degeneration as facial nerve lesions elicited by ricin injection, which produce neuronal death without regeneration, fail to augment FKBP-12 expression in the facial nucleus. The time course for accumulation of FKBP-12 in sciatic nerve segments following nerve crush indicates rapid axonal transport at a rate similar to GAP-43.