PT  - JOURNAL ARTICLE
AU  - GB Vannelli
AU  - F Ensoli
AU  - R Zonefrati
AU  - Y Kubota
AU  - A Arcangeli
AU  - A Becchetti
AU  - G Camici
AU  - T Barni
AU  - CJ Thiele
AU  - GC Balboni
TI  - Neuroblast long-term cell cultures from human fetal olfactory epithelium respond to odors
AID  - 10.1523/JNEUROSCI.15-06-04382.1995
DP  - 1995 Jun 01
TA  - The Journal of Neuroscience
PG  - 4382--4394
VI  - 15
IP  - 6
4099  - http://www.jneurosci.org/content/15/6/4382.short
4100  - http://www.jneurosci.org/content/15/6/4382.full
SO  - J. Neurosci.1995 Jun 01; 15
AB  - Primary cell cultures from human fetal olfactory neuroepithelium have been isolated, cloned, and propagated in continuous in vitro culture for approximately 1 year. The two clones we report here synthesize both neuronal proteins and olfactory-specific markers as well as the putative olfactory neurotransmitter, carnosine. In addition, patchclamp experiments reveal that these cells are electrically excitable. Following exposure to a panel of aromatic chemicals one of the cell cultures shows a specific increase in intracellular cAMP, indicating that some degree of functional maturity is expressed in vitro. The results suggest that these cells originate from the “stem cell” compartment that gives rise to mature olfactory receptor neurons. These long-term cell cultures represent models that will be useful in studying the mechanism(s) of olfaction and the regulation of olfactory neurogenesis and differentiation.