RT Journal Article
SR Electronic
T1 Induction of interleukin-6 in axotomized sensory neurons
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 5130
OP 5138
DO 10.1523/JNEUROSCI.15-07-05130.1995
VO 15
IS 7
A1 Murphy, PG
A1 Grondin, J
A1 Altares, M
A1 Richardson, PM
YR 1995
UL http://www.jneurosci.org/content/15/7/5130.abstract
AB RNA from rat dorsal root ganglia was analyzed in search of potentially beneficial cytokines that are induced in dorsal root ganglia by nerve injury. By reverse transcription, the PCR, and Southern blotting, interleukin-6 mRNA was detected during development but not in normal adult dorsal root ganglia, reappeared within 1 d of sciatic nerve transection, was maximally increased after 2 and 4 d, and decreased below the threshold of detection within 1 week. By RNase protection assay, interleukin-6 mRNA was consistently present in RNA from dorsal root ganglia removed from rats 4 d following transection but not in control dorsal root ganglia. Interleukin-6 bioactivity was also present in dorsal root ganglia following nerve injury. By in situ hybridization, interleukin-6 mRNA was localized within large and medium- sized axotomized neurons. In summary, some sensory neurons respond to axotomy with a brisk transient increase in synthesis of interleukin-6. Injury to the sciatic nerve also induced mRNAs for interleukin-1 beta and tumor necrosis factor-alpha in dorsal root ganglia. The inductions of interleukin-1 beta and tumor necrosis factor-alpha mRNAs were later and more sustained than that of interleukin-6 mRNA. The cellular sources of these two cytokines have not been defined.