RT Journal Article
SR Electronic
T1 Neuroligand-evoked calcium-dependent release of excitatory amino acids from Schwann cells
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 5831
OP 5839
DO 10.1523/JNEUROSCI.15-08-05831.1995
VO 15
IS 8
A1 Parpura, V
A1 Liu, F
A1 Jeftinija, KV
A1 Haydon, PG
A1 Jeftinija, SD
YR 1995
UL http://www.jneurosci.org/content/15/8/5831.abstract
AB Bradykinin caused a receptor-mediated increase in release of the excitatory amino acids (EAAs) glutamate and aspartate from Schwann cell cultures obtained from dorsal root ganglia (DRG) together with an increase in the cytoplasmic level of free calcium. Perturbations which inhibited brady-kinin-induced calcium mobilization prevented the release of EAAs from glia. The addition of ionomycin caused a calcium- dependent release of EAAs. Therefore, bradykinin causes calcium dependent-release of EAAs from DRG Schwann cells. Bradykinin did not cause cell swelling and p-chloromercuriphenylsulfonic acid, an inhibitor of the electrogenic glutamate transporter, did not reduce bradykinin-induced EAA release. Therefore, bradykinin stimulates EAA release from Schwann cells through a mechanism that is neither the previously described volume regulated release mechanism nor due to the reversal of the glutamate transporter.