TY - JOUR T1 - Cell Type-Specific Sorting of Neuropeptides: A Mechanism to Modulate Peptide Composition of Large Dense-Core Vesicles JF - The Journal of Neuroscience JO - J. Neurosci. SP - 7930 LP - 7940 DO - 10.1523/JNEUROSCI.16-24-07930.1996 VL - 16 IS - 24 AU - Judith Klumperman AU - Sabine Spijker AU - Jan van Minnen AU - Hilary Sharp-Baker AU - August B. Smit AU - Wijnand P. M. Geraerts Y1 - 1996/12/15 UR - http://www.jneurosci.org/content/16/24/7930.abstract N2 - The CNS of Lymnaea stagnalis contains two populations of egg-laying hormone (ELH)-producing neurons that differ in size and topology. In type I neurons, all peptides located C-terminally from the cleavage site Arg-Ser-Arg-Arg180–183are sorted into secretory large dense-core vesicles (LDCV), whereas N-terminal-located peptides accumulate in a distinct type of vesicle, the large electrondense granule (LEG). Via immunoelectron microscopy, we now show that the second population of ELH-producing neurons, type II neurons, lack LEG and incorporate all proELH-derived peptides into LDCV. This finding provides the first example of a cell type-specific sorting of neuropeptides into LDCV. Furthermore, we provide evidence that LEG are formed through a differential condensation process in thetrans-Golgi network and that these bodies are ultimately degraded. Analysis of the endoprotease composition of the two types of proELH-producing neurons suggests that the formation of LEG, and consequently the retention of N-terminal peptides from the secretory pathway, requires the action of a furin-like protein. ER -