RT Journal Article
SR Electronic
T1 Inositol 1,4,5-Trisphosphate-Gated Calcium Transport through Plasma Membranes in Nerve Terminals
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 2891
OP 2900
DO 10.1523/JNEUROSCI.16-09-02891.1996
VO 16
IS 9
A1 Hiroshi Ueda
A1 Shigeki Tamura
A1 Nobuyuki Fukushima
A1 Toshiaki Katada
A1 Michio Ui
A1 Masamichi Satoh
YR 1996
UL http://www.jneurosci.org/content/16/9/2891.abstract
AB We developed new biochemical approaches to demonstrate the presence of inositol 1,4,5-trisphosphate (InsP3)-gated calcium channels in presynaptic plasma membranes (SPM) and their involvement in the presynaptic receptor-mediated Ca2+ influx into nerve terminals. In perfusion experiments using SPM vesicles preloaded with45Ca2+, InsP3 elicited the release of45Ca2+ into perfusates in a saturable manner. The InsP3-evoked45Ca2+ release from resealed SPM vesicles was more potent than that from resealed vesicles using any other subcellular fractions. Here we also report the involvement of InsP3-gated mechanisms in the presynaptic receptor-mediated Ca2+ influx into synaptosomes (nerve terminals) by use of such resealed vesicles reconstituted with purified Gi1.