RT Journal Article
SR Electronic
T1 A New Form of Inherited Red-Blindness Identified in Zebrafish
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 4236
OP 4242
DO 10.1523/JNEUROSCI.17-11-04236.1997
VO 17
IS 11
A1 Brockerhoff, Susan E.
A1 Hurley, James B.
A1 Niemi, Gregory A.
A1 Dowling, John E.
YR 1997
UL http://www.jneurosci.org/content/17/11/4236.abstract
AB A red-blind zebrafish mutant, partial optokinetic response b (pob), has been isolated by measuring eye movements of larvae in a three-generation screen for recessive mutations affecting the visual system. pob larvae exhibit eye movements in response to rotating black and white stripes illuminated with white light, but they do not move their eyes when the stripes are illuminated with red light. Physiological, immunohistochemical, andin situ hybridization analyses of pobretinas showed a selective loss of red-sensitive cones at 5 days postfertilization (dpf). At 3 dpf, cells expressing red opsin are present, suggesting that red-sensitive cones form initially but then disappear rapidly, whereas other photoreceptors remain. Linkage analysis indicated that the mutation identified in thepob mutant is not at the red opsin locus. Because red opsin is the only known molecule unique to red cones, these data suggest that a novel gene is required for the maintenance or function of red cones.