PT - JOURNAL ARTICLE AU - Ranjita Betarbet AU - Robert Turner. AU - Vijay Chockkan AU - Mahlon R. DeLong AU - Kelly A. Allers AU - Judith Walters AU - Allan I. Levey AU - J. Timothy Greenamyre TI - Dopaminergic Neurons Intrinsic to the Primate Striatum AID - 10.1523/JNEUROSCI.17-17-06761.1997 DP - 1997 Sep 01 TA - The Journal of Neuroscience PG - 6761--6768 VI - 17 IP - 17 4099 - http://www.jneurosci.org/content/17/17/6761.short 4100 - http://www.jneurosci.org/content/17/17/6761.full SO - J. Neurosci.1997 Sep 01; 17 AB - Intrinsic, striatal tyrosine hydroxylase-immunoreactive (TH-i) cells have received little consideration. In this study we have characterized these neurons and their regulatory response to nigrostriatal dopaminergic deafferentation. TH-i cells were observed in the striatum of both control and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated monkeys; TH-i cell counts, however, were 3.5-fold higher in the striatum of MPTP-lesioned monkeys. To establish the dopaminergic nature of the TH-i cells, sections were double-labeled with antibodies to dopamine transporter (DAT). Immunofluorescence studies demonstrated that nearly all TH-i cells were double-labeled with DAT, suggesting that they contain the machinery to be functional dopaminergic neurons. Two types of TH-i cells were identified in the striatum: small, aspiny, bipolar cells with varicose dendrites and larger spiny, multipolar cells. The aspiny cells, which were more prevalent, corresponded morphologically to the GABAergic interneurons of the striatum. Double-label immunofluorescence studies using antibodies to TH and glutamate decarboxylase (GAD67), the synthetic enzyme for GABA, showed that 99% of the TH-i cells were GAD67-positive. Very few (<1%) of the TH-i cells, however, were immunoreactive for the calcium-binding proteins calbindin and parvalbumin. In summary, these results demonstrate that the dopaminergic cell population of the striatum responds to dopamine denervation by increasing in number, apparently to compensate for loss of extrinsic dopaminergic innervation. Moreover, this population of cells corresponds largely with the intrinsic GABAergic cells of the striatum. This study also suggests that the adult primate striatum does retain some intrinsic capacity to compensate for dopaminergic cell loss.