TY - JOUR T1 - 5HT<sub>4</sub> Receptors Couple Positively to Tetrodotoxin-Insensitive Sodium Channels in a Subpopulation of Capsaicin-Sensitive Rat Sensory Neurons JF - The Journal of Neuroscience JO - J. Neurosci. SP - 7181 LP - 7189 DO - 10.1523/JNEUROSCI.17-19-07181.1997 VL - 17 IS - 19 AU - Carla G. Cardenas AU - Lucinda P. Del Mar AU - Brian Y. Cooper AU - Reese S. Scroggs Y1 - 1997/10/01 UR - http://www.jneurosci.org/content/17/19/7181.abstract N2 - The distribution of tetrodotoxin (TTX)-sensitive and -insensitive Na+ currents and their modulation by serotonin (5HT) and prostaglandin E2 (PGE2) was studied in four different types of dorsal root ganglion (DRG) cell bodies (types 1, 2, 3, and 4), which were previously identified on the basis of differences in membrane properties (Cardenas et al., 1995). Types 1 and 2 DRG cells expressed TTX-insensitive Na+currents, whereas types 3 and 4 DRG cells exclusively expressed TTX-sensitive Na+ currents.Application of 5HT (1–10 μm) increased TTX-insensitive Na+ currents in type 2 DRG cells but did not affect Na+ currents in type 1, 3, or 4 DRG cells. The 5HT receptor involved resembled the 5HT4 subtype. It was activated by 5-methoxy-N,N-dimethyltryptamine (10 μm) but not by 5-carboxyamidotryptamine (1 μm), (+)-8-hydroxydipropylaminotetralin (10 μm), or 2-methyl-5HT (10 μm), and was blocked by ICS 205-930 with an EC50 of ∼2 μm but not by ketanserin (1 μm). PGE2 (4 or 10 μm) also increased Na+ currents in varying portions of cells in all four groups.The effect of 5HT and PGE2 on Na+currents was delayed for 20–30 sec after exposure to 5HT, suggesting the involvement of a cytosolic diffusible component in the signaling pathway. The agonist-mediated increase in Na+current, however, was not mimicked by 8-chlorophenylthio-cAMP (200 μm), suggesting the possibility that cAMP was not involved.The data suggest that the 5HT- and PGE2-mediated increase in Na+ current may be involved in hyperesthesia in different but overlapping subpopulations of nociceptors. ER -