TY - JOUR T1 - Effects of Sleep on Wake-Induced c-<em>fos</em> Expression JF - The Journal of Neuroscience JO - J. Neurosci. SP - 9746 LP - 9750 DO - 10.1523/JNEUROSCI.17-24-09746.1997 VL - 17 IS - 24 AU - Radhika Basheer AU - Jonathan E. Sherin AU - Clifford B. Saper AU - James I. Morgan AU - Robert W. McCarley AU - Priyattam J. Shiromani Y1 - 1997/12/15 UR - http://www.jneurosci.org/content/17/24/9746.abstract N2 - We investigated the effects of sleep on wake-induced c-fos expression in the cerebral cortex of rats and c-fos-lacZ transgenic mice. In the cortex of rats, the levels of c-Fos, detected both by immunocytochemistry and Western blot, remained high during 6 or 12 hr of enforced wakefulness but declined rapidly (within 1 hr) with increasing time of recovery sleep. Similarly, in the transgenic mice in which lacZexpression is driven from the c-fos promoter, β-galactosidase activity was high after enforced wakefulness and declined with increasing amounts of sleep. These results suggest that the decrease in c-Fos protein in cortical neurons during sleep may be attributable to cessation of c-fos expression, activation of a process that degrades the wake-induced c-Fos, or both. ER -