RT Journal Article
SR Electronic
T1 Temporal and Spatial Expression Patterns of Transgenes Containing Increasing Amounts of the Drosophila Clock Geneperiod and a lacZ Reporter: Mapping Elements of the PER Protein Involved in Circadian Cycling
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 676
OP 696
DO 10.1523/JNEUROSCI.17-02-00676.1997
VO 17
IS 2
A1 Ralf Stanewsky
A1 Brigitte Frisch
A1 Christian Brandes
A1 Melanie J. Hamblen-Coyle
A1 Michael Rosbash
A1 Jeffrey C. Hall
YR 1997
UL http://www.jneurosci.org/content/17/2/676.abstract
AB Rhythmic oscillations of the PER protein, the product of theDrosophila period (per) gene, in brain neurons of the adult fly are strongly involved in the control of circadian rhythms. We analyzed temporal and spatial expression patterns of three per–reporter fusion genes, which share the same 4 kb regulatory upstream region but contain increasing amounts ofper’s coding region fused in frame to the bacteriallacZ gene. The fusion proteins contained either the N-terminal half (SG), the N-terminal two-thirds (BG), or nearly all (XLG) of the PER protein. All constructs led to reporter signals only in the known per-expressing cell types within the anterior CNS and PNS. Whereas the staining intensity of SG flies was constantly high at different Zeitgeber times, the in situ signals in BG and XLG flies cycled with ∼24 hr periodicity in the PER-expressing brain cells in wild-type andper01 loss of function flies. Despite the rhythmic fusion-gene expression within the relevant neurons ofper01 BG flies, their locomotor activity in light/dark cycling conditions and in constant darkness was identical to that of per01 controls, uncoupling protein cycling from rhythmic behavior. The XLG construct restored weak behavioral rhythmicity to (otherwise)per01 flies, indicating that the C-terminal third of PER (missing in BG) is necessary to fulfill the biological function of this clock protein.