RT Journal Article
SR Electronic
T1 The seizure Locus Encodes the DrosophilaHomolog of the HERG Potassium Channel
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 882
OP 890
DO 10.1523/JNEUROSCI.17-03-00882.1997
VO 17
IS 3
A1 XinJing Wang
A1 Elaine R. Reynolds
A1 Péter Déak
A1 Linda M. Hall
YR 1997
UL http://www.jneurosci.org/content/17/3/882.abstract
AB Mutations in the seizure (sei) locus cause temperature-induced hyperactivity, followed by paralysis. Gene cloning studies have established that the seizuregene product is the Drosophila homolog ofHERG, a member of the eag family of K+ channels implicated in one form of hereditary long QT syndrome in humans. A series of five null alleles with premature stop codons are all recessive, but viable. A missense mutation in thesei gene, which changes the charge at a conserved glutamate residue near the outer mouth of the pore, has a semidominant phenotype, suggesting that the mutant seizure protein acts as a poison in a multimeric complex. Transformation rescue of a null allele with a cDNA under the control of an inducible promoter demonstrates that induced expression of seizure potassium channels in adults rescues the paralytic phenotype. This rescue decays with at1/2 of ∼1-1.5 d after gene induction is discontinued, providing the first estimate of ion channel stability in an intact, multicellular animal.