RT Journal Article
SR Electronic
T1 Mosaics of Islet-1-Expressing Amacrine Cells Assembled by Short-Range Cellular Interactions
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 7831
OP 7838
DO 10.1523/JNEUROSCI.17-20-07831.1997
VO 17
IS 20
A1 Lucia Galli-Resta
A1 Giovanni Resta
A1 Seong-Seng Tan
A1 Benjamin E. Reese
YR 1997
UL http://www.jneurosci.org/content/17/20/7831.abstract
AB The nervous system has a modular architecture with neurons of the same type commonly organized in nonrandom arrays or mosaics. Modularity is essential to parallel processing of sensory information and has provided a key element for brain evolution, but we still know very little of the way neuronal mosaics form during development. Here we have identified the immature elements of two retinal mosaics, the choline acetyltransferase (ChAT) amacrine cells, by their early expression of the homeodomain protein Islet-1, and we show that spatial ordering is an intrinsic property of the two Islet-1 mosaics, dynamically maintained while new elements are inserted into the mosaics. Migrating Islet-1 cells do not show this spatial ordering, indicating that they must move tangentially as they enter the mosaic, under the action of local mechanisms. Clonal territory analysis in X-inactivation transgenic mice confirms the lateral displacement of ChAT amacrine cells away from their clonal columns of origin, and mathematical models show how short-range cellular interactions can guide the assemblage of these mosaics via a simple biological rule.