TY - JOUR T1 - Effects of Glucose Deprivation, Chemical Hypoxia, and Simulated Ischemia on Na<sup>+</sup> Homeostasis in Rat Spinal Cord Astrocytes JF - The Journal of Neuroscience JO - J. Neurosci. SP - 3554 LP - 3562 DO - 10.1523/JNEUROSCI.18-10-03554.1998 VL - 18 IS - 10 AU - Christine R. Rose AU - Stephen G. Waxman AU - Bruce R. Ransom Y1 - 1998/05/15 UR - http://www.jneurosci.org/content/18/10/3554.abstract N2 - A steep inwardly directed Na+ gradient is essential for glial functions such as glutamate reuptake and regulation of intracellular ion concentrations. We investigated the effects of glucose deprivation, chemical hypoxia, and simulated ischemia on intracellular Na+ concentration ([Na+]i) in cultured spinal cord astrocytes using fluorescence ratio imaging with sodium-binding benzofuran isophthalate (SBFI) AM. Glucose removal or chemical hypoxia (induced by 10 mm NaN3) for 60 min increased [Na+]i from a baseline of 8.3 to 11 mm. Combined glycolytic and respiratory blockage by NaN3 and 0 glucose saline caused [Na+]i to increase by 20 mm, similar to the [Na+]iincreases elicited by blocking the Na+/K+-ATPase with ouabain. Recovery from large [Na+]i increases (&gt;15 mm) induced by the glutamatergic agonist kainate was attenuated during glucose deprivation or NaN3 application and was blocked in NaN3 and 0 glucose. To mimic in vivo ischemia, we exposed astrocytes to NaN3 and 0 glucose saline containing l-lactate and glutamate with increased [K+] and decreased [Na+], [Ca2+], and pH. This induced an [Na+]i decrease followed by an [Na+]i rise and a further [Na+]i increase after reperfusion with standard saline. Similar multiphasic [Na+]i changes were observed after NaN3 and 0 glucose saline with only reduced [Na+]e. Our results suggest that the ability to maintain a low [Na+]ienables spinal cord astrocytes to continue uptake of K+ and/or glutamate at the onset of energy failure. With prolonged energy failure, however, astrocytic [Na+]i rises; with loss of their steep transmembrane Na+ gradient, astrocytes may aggravate metabolic insults by carrier reversal and release of acid, K+, and/or glutamate into the extracellular space. ER -