RT Journal Article
SR Electronic
T1 Differential Intracellular Sorting of Immediate Early Gene mRNAs Depends on Signals in the mRNA Sequence
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 26
OP 35
DO 10.1523/JNEUROSCI.18-01-00026.1998
VO 18
IS 1
A1 Christopher S. Wallace
A1 Gregory L. Lyford
A1 Paul F. Worley
A1 Oswald Steward
YR 1998
UL http://www.jneurosci.org/content/18/1/26.abstract
AB This study characterizes the differential targeting of recently synthesized immediate early gene (IEG) mRNAs to neuronal cell bodies versus dendrites and tests the hypothesis that this targeting is based on signals in the encoded proteins. A single electroconvulsive seizure induces the expression of a number of IEG mRNAs in granule cells of the dentate gyrus. Most of these IEG mRNAs remain in the cell body, including two that are characterized in the present study (the mRNAs for NGFI-A and COX-2). In contrast, the mRNA for Arcmoved rapidly into dendrites at an apparent rate of ∼300 μm/hr. Inhibiting protein synthesis with cycloheximide did not disrupt the differential mRNA sorting, demonstrating that the differential targeting of mRNAs is not dependent on translation.