PT  - JOURNAL ARTICLE
AU  - Takafumi Inoue
AU  - Kunio Kato
AU  - Kazuhisa Kohda
AU  - Katsuhiko Mikoshiba
TI  - Type 1 Inositol 1,4,5-Trisphosphate Receptor Is Required for Induction of Long-Term Depression in Cerebellar Purkinje Neurons
AID  - 10.1523/JNEUROSCI.18-14-05366.1998
DP  - 1998 Jul 15
TA  - The Journal of Neuroscience
PG  - 5366--5373
VI  - 18
IP  - 14
4099  - http://www.jneurosci.org/content/18/14/5366.short
4100  - http://www.jneurosci.org/content/18/14/5366.full
SO  - J. Neurosci.1998 Jul 15; 18
AB  - The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that releases Ca2+ from internal Ca2+ stores in response to InsP3. Although InsP3R is highly expressed in various regions of the mammalian brain, the functional role of this receptor has not been clarified. We show here that cerebellar slices prepared from mice with a disrupted InsP3R type 1 gene, which is predominantly expressed in Purkinje cells, completely lack long-term depression (LTD), a model of synaptic plasticity in the cerebellum. Moreover, a specific antibody against InsP3R1, introduced into wild-type Purkinje cells through patch pipettes, blocked the induction of LTD. These data indicate that, in addition to Ca2+ influx through Ca2+ channels on the plasma membrane, Ca2+ release from InsP3R plays an essential role in the induction of LTD, suggesting a physiological importance for InsP3R in Purkinje cells.