PT  - JOURNAL ARTICLE
AU  - Kelly Kruger
AU  - Angie S. Tam
AU  - Cynthia Lu
AU  - David W. Sretavan
TI  - Retinal Ganglion Cell Axon Progression from the Optic Chiasm to Initiate Optic Tract Development Requires Cell Autonomous Function of GAP-43
AID  - 10.1523/JNEUROSCI.18-15-05692.1998
DP  - 1998 Aug 01
TA  - The Journal of Neuroscience
PG  - 5692--5705
VI  - 18
IP  - 15
4099  - http://www.jneurosci.org/content/18/15/5692.short
4100  - http://www.jneurosci.org/content/18/15/5692.full
SO  - J. Neurosci.1998 Aug 01; 18
AB  - Pathfinding mechanisms underlying retinal ganglion cell (RGC) axon growth from the optic chiasm into the optic tract are unknown. Previous work has shown that mouse embryos deficient in GAP-43 have an enlarged optic chiasm within which RGC axons were reportedly stalled. Here we have found that the enlarged chiasm of GAP-43 null mouse embryos appears subsequent to a failure of the earliest RGC axons to progress laterally through the chiasm–tract transition zone to form the optic tract. Previous work has shown that ventral diencephalon CD44/stage-specific embryonic antigen (SSEA) neurons provide guidance information for RGC axons during chiasm formation. Here we found that in the chiasm–tract transition zone, axons of CD44/SSEA neurons precede RGC axons into the lateral diencephalic wall and like RGC axons also express GAP-43. However unlike RGC axons, CD44/SSEA axon trajectories are unaffected in GAP-43 null embryos, indicating that GAP-43–dependent guidance at this site is RGC axon specific or occurs only at specific developmental times. To determine whether the phenotype results from loss of GAP-43 in RGCs or in diencephalon components such as CD44/SSEA axons, wild-type, heterozygous, or homozygous GAP-43 null donor retinal tissues were grafted onto host diencephalons of all three genotypes, and graft axon growth into the optic tract region was assessed. Results show that optic tract development requires cell autonomous GAP-43 function in RGC axons and not in cellular elements of the ventral diencephalon or transition zone.