@article {Wang7372, author = {Xin Wang and Stanley W. Halvorsen}, title = {Reciprocal Regulation of Ciliary Neurotrophic Factor Receptors and Acetylcholine Receptors during Synaptogenesis in Embryonic Chick Atria}, volume = {18}, number = {18}, pages = {7372--7380}, year = {1998}, doi = {10.1523/JNEUROSCI.18-18-07372.1998}, publisher = {Society for Neuroscience}, abstract = {Ciliary neurotrophic factor (CNTF) has been implicated in the development, survival, and maintenance of a broad range of neurons and glia in the peripheral nervous system and the CNS. Evidence also suggests that CNTF may affect development of cells outside the nervous system. We have found that functional CNTF and its receptor are expressed in developing embryonic chick heart and may be involved in parasympathetic synapse formation. CNTF and CNTF receptor mRNA levels were highest at embryonic day 11 (E11){\textendash}E13, the period of parasympathetic innervation in chick atria. Levels of atrial CNTF receptor mRNA were fourfold greater at E13 than at E6 and at E13 were 2.5-fold higher in atria than in ventricle, corresponding to the higher degree of parasympathetic innervation occurring in atria. Treatment of isolated atria or cultured atrial myocytes with recombinant human or avian CNTF resulted in the tyrosine phosphorylation and nuclear translocation of the signal transducer and activator of transcription STAT3. The developmental increase in atrial CNTF receptor mRNA was enhanced by stimulating muscarinic receptors with carbacholin ovo and was inhibited by blocking muscarinic cholinergic receptors with atropine. Treatment of cultured atrial myocytes with CNTF resulted in a twofold increase in the levels of muscarinic receptors. Thus, CNTF was able to regulate a key component of parasympathetic synapses on atrial myocytes. These results suggest a postsynaptic role for CNTF in the onset of parasympathetic function in the developing heart and provide new clues to molecular mechanisms directing synapse formation at targets of the autonomic nervous system.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/18/18/7372}, eprint = {https://www.jneurosci.org/content/18/18/7372.full.pdf}, journal = {Journal of Neuroscience} }