TY - JOUR T1 - Chronic Pain is Associated with Increased TrkA Immunoreactivity in Spinoreticular Neurons JF - The Journal of Neuroscience JO - J. Neurosci. SP - 5482 LP - 5492 DO - 10.1523/JNEUROSCI.19-13-05482.1999 VL - 19 IS - 13 AU - Sophie Pezet AU - Brigitte Onténiente AU - Gaël Grannec AU - Bernard Calvino Y1 - 1999/07/01 UR - http://www.jneurosci.org/content/19/13/5482.abstract N2 - Repetitive noxious stimulation leads to permanent adaptive changes of central pathways involved in the genesis and integration of nociception. Several classes of neurotrophic factors that affect brain plasticity are also involved in the regulation of sensory functions in adulthood. To investigate a putative role of nerve growth factor (NGF) in central plasticity linked to chronic pain, modifications in immunoreactivity (IR) for the high-affinity NGF receptor, TrkA, were studied at spinal levels in a rat model of inflammatory chronic pain, adjuvant-induced arthritis (AIA). We report a specific increase in the number of TrkA-IR profiles in laminae V–VI at lumbar levels L3 and L4 in arthritic rats. Tract tracing using FluoroGold injections in the ventrobasal complex of the thalamus and in the brainstem showed that these increased TrkA-IR profiles are spinoreticular neurons. Dual labeling with calcitonin gene-related peptide or substance P showed that TrkA-IR neurons were mainly located in projection fields of small- to medium-sized primary afferent fibers, which convey nociceptive inputs. These results suggest that TrkA-containing neurons of the spinal dorsal horn participate in the first central relay of transmission of nociceptive information to supraspinal centers. Enhanced numbers of TrkA-IR neurons during AIA strongly support the hypothesis of a participation of NGF in adaptive mechanisms of central nociceptive pathways observed in chronic pain states. ER -