RT Journal Article
SR Electronic
T1 Knock-Out of the Neural Death Effector Domain Protein PEA-15 Demonstrates That Its Expression Protects Astrocytes from TNFα-Induced Apoptosis
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 8244
OP 8251
DO 10.1523/JNEUROSCI.19-19-08244.1999
VO 19
IS 19
A1 Daniel Kitsberg
A1 Etienne Formstecher
A1 Mireille Fauquet
A1 Miroslav Kubes
A1 Jocelyne Cordier
A1 Brigitte Canton
A1 GuoHua Pan
A1 Malvyne Rolli
A1 Jacques Glowinski
A1 Hervé Chneiweiss
YR 1999
UL http://www.jneurosci.org/content/19/19/8244.abstract
AB Apoptosis is a very general phenomenon, but only a few reports concern astrocytes. Indeed, astrocytes express receptors for tumor necrosis factor (TNF) α, a cytokine demonstrated on many cells and tissues to mediate apoptosis after recruitment of adaptor proteins containing a death effector domain (DED). PEA-15 is a DED-containing protein prominently expressed in the CNS and particularly abundant in astrocytes. This led us to investigate if PEA-15 expression could be involved in astrocytic protection against deleterious effects of TNF. In vitro assays evidence that PEA-15 may bind to DED-containing protein FADD and caspase-8 known to be apical adaptors of the TNF apoptotic signaling. After generation of PEA-15 null mutant mice, our results demonstrate that PEA-15 expression increases astrocyte survival after exposure to TNF.