PT  - JOURNAL ARTICLE
AU  - Ohki-Hamazaki, Hiroko
AU  - Sakai, Yasushi
AU  - Kamata, Katsuo
AU  - Ogura, Hiroo
AU  - Okuyama, Shigeru
AU  - Watase, Kei
AU  - Yamada, Kazuyuki
AU  - Wada, Keiji
TI  - Functional Properties of Two Bombesin-Like Peptide Receptors Revealed by the Analysis of Mice Lacking Neuromedin B Receptor
AID  - 10.1523/JNEUROSCI.19-03-00948.1999
DP  - 1999 Feb 01
TA  - The Journal of Neuroscience
PG  - 948--954
VI  - 19
IP  - 3
4099  - http://www.jneurosci.org/content/19/3/948.short
4100  - http://www.jneurosci.org/content/19/3/948.full
SO  - J. Neurosci.1999 Feb 01; 19
AB  - The neuromedin B-preferring receptor (NMB-R) is one of the members of the bombesin (BN)-like peptide receptor subfamily in mammals. Previously, we have generated and characterized mice with targeted disruption of the two other BN-like peptide receptors, bombesin receptor subtype-3 (BRS-3) and gastrin-releasing peptide-preferring receptor (GRP-R). Here we describe the generation and analysis of NMB-R-deficient mice to investigate how NMB-R differs from BRS-3 and GRP-R. Compensation for NMB-R deficiency by overexpression of GRP-R and/or BRS-3 was not detected. Although the hypothermic effect of NMB was reduced by 50% in NMB-R-deficient mice, the effect of GRP infusion was comparable to the wild-type mice. In contrast, fundic smooth muscle contraction on stimulation with NMB or GRP was normal in NMB-R-deficient mice. Administration of GRP but not NMB suppressed glucose intake in both normal and NMB-R-deficient mice. These results suggest that the NMB-R has an essential role in thermoregulation, but not for smooth muscle contraction of the fundus or for the suppression of feeding behavior. In addition, the behavioral phenotypes of GRP-R-deficient mice were not observed in NMB-R-deficient mice. These data show that the functions of NMB-R and GRP-R are distinct, with only partial overlap.