PT  - JOURNAL ARTICLE
AU  - Zhu, Wei Jian
AU  - Roper, Steven N.
TI  - Reduced Inhibition in an Animal Model of Cortical Dysplasia
AID  - 10.1523/JNEUROSCI.20-23-08925.2000
DP  - 2000 Dec 01
TA  - The Journal of Neuroscience
PG  - 8925--8931
VI  - 20
IP  - 23
4099  - http://www.jneurosci.org/content/20/23/8925.short
4100  - http://www.jneurosci.org/content/20/23/8925.full
SO  - J. Neurosci.2000 Dec 01; 20
AB  - Cortical dysplasia has a strong association with epilepsy in humans, but the underlying mechanisms for this are poorly understood.In utero irradiation of rats produces diffuse cortical dysplasia and neuronal heterotopia in the neocortex and hippocampus. Using in vitro neocortical slices, whole-cell patch-clamp recordings were obtained from pyramidal neurons in dysplastic cortex and control neocortex. Spontaneous IPSCs were reduced in amplitude (35%) and frequency (70%) in pyramidal cells from dysplastic cortex. Miniature IPSCs were reduced in frequency (66%) in dysplastic cortex. Two additional measures of cortical inhibition, monosynaptic evoked IPSCs and paired pulse depression of evoked EPSCs, were also impaired in dysplastic cortex. Spontaneous EPSCs were increased in amplitude (42%) and frequency (77%) in dysplastic cortex, but miniature EPSCs were not different between the two groups. These data demonstrate significant physiological impairment in inhibitory synaptic transmission in experimental cortical dysplasia. This supports previous immunohistochemical findings in this model and observations in humans of a reduction in the density of inhibitory interneurons in dysplastic cortex.