TY - JOUR T1 - Schwann Cells Are Removed from the Spinal Cord after Effecting Recovery from Paraplegia JF - The Journal of Neuroscience JO - J. Neurosci. SP - 9215 LP - 9223 DO - 10.1523/JNEUROSCI.20-24-09215.2000 VL - 20 IS - 24 AU - Luc Jasmin AU - Gabriella Janni AU - Theodore M. Moallem AU - Douglas A. Lappi AU - Peter T. Ohara Y1 - 2000/12/15 UR - http://www.jneurosci.org/content/20/24/9215.abstract N2 - Remyelination of the CNS is necessary to restore neural function in a number of demyelinating conditions. Schwann cells, the myelinating cells of the periphery, are candidates for this purpose because they have more robust regenerative properties than their central homologs, the oligodendrocytes. Although the ability of Schwann cells to remyelinate the CNS has been demonstrated, their capacity to enter the adult spinal cord in large numbers and effect functional recovery remains uncertain. We used cholera toxin B-subunit conjugated to saporin to demyelinate the rat lumbar spinal cord, remove macroglia, and produce paraplegia. After the removal of oligodendrocyte and astrocyte debris by invading macrophages, there was a spontaneous entry of Schwann cells into the spinal cord, along with axonal remyelination and concomitant functional recovery from paraplegia occurring within 75 d. The Schwann cells appeared to enter the dorsal funiculi via the dorsal root entry zone and the lateral funiculi via rootlets that had become adherent to the lateral spinal cord after the inflammation. In the following weeks, Schwann cell myelin surrounding central axons was progressively replaced by oligodendrocyte myelin without lapse in motor function. Our results show that endogenous Schwann cells can reverse a severe neurological deficit caused by CNS demyelination and enable later oligodendrocyte remyelination. ER -