@article {Arancio143, author = {Ottavio Arancio and Irina Antonova and Stepan Gambaryan and Suzanne M. Lohmann and Jason S. Wood and David S. Lawrence and Robert D. Hawkins}, title = {Presynaptic Role of cGMP-Dependent Protein Kinase during Long-Lasting Potentiation}, volume = {21}, number = {1}, pages = {143--149}, year = {2001}, doi = {10.1523/JNEUROSCI.21-01-00143.2001}, publisher = {Society for Neuroscience}, abstract = {Previous research has suggested that cGMP-dependent protein kinases (cGKs) may play a role in long-term potentiation in hippocampus, but their site of action has been unknown. We examined this question at synapses between pairs of hippocampal neurons in dissociated cell culture. Injection of a specific peptide inhibitor of cGK into the presynaptic but not the postsynaptic neuron blocked long-lasting potentiation induced by tetanic stimulation of the presynaptic neuron. As controls, injection of a scrambled peptide or a peptide inhibitor of cAMP-dependent protein kinase into either neuron did not block potentiation. Conversely, injection of the α isozyme of cGK type I into the presynaptic but not the postsynaptic neuron produced activity-dependent potentiation that did not require NMDA receptor activation. Evidence from Western blots, reverse transcription-PCR, activity assays, and immunocytochemistry indicates that endogenous cGK type I is present in the neurons, including presynaptic terminals. These results support the idea that cGK plays an important presynaptic role during the induction of long-lasting potentiation in hippocampal neurons.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/21/1/143}, eprint = {https://www.jneurosci.org/content/21/1/143.full.pdf}, journal = {Journal of Neuroscience} }