PT  - JOURNAL ARTICLE
AU  - Mats H. Holmqvist
AU  - Jie Cao
AU  - Maria H. Knoppers
AU  - Mark E. Jurman
AU  - Peter S. Distefano
AU  - Kenneth J. Rhodes
AU  - Yu Xie
AU  - W. Frank An
TI  - Kinetic Modulation of Kv4-Mediated A-Current by Arachidonic Acid Is Dependent on Potassium Channel Interacting Proteins
AID  - 10.1523/JNEUROSCI.21-12-04154.2001
DP  - 2001 Jun 15
TA  - The Journal of Neuroscience
PG  - 4154--4161
VI  - 21
IP  - 12
4099  - http://www.jneurosci.org/content/21/12/4154.short
4100  - http://www.jneurosci.org/content/21/12/4154.full
SO  - J. Neurosci.2001 Jun 15; 21
AB  - The Kv4 subfamily of voltage-gated potassium channels is responsible for the transient A-type potassium current that operates at subthreshold membrane potentials to control membrane excitability. Arachidonic acid was shown recently to modulate both the peak amplitude and kinetics of the hippocampal A-current. However, inXenopus oocytes, arachidonic acid only inhibited the peak amplitude of Kv4 current without modifying its kinetics. These results suggest the existence of Kv4 auxiliary subunit(s) in native cells. We report here a K-channel interacting protein (KChIP)-dependent kinetic modulation of Kv4.2 current in Chinese hamster ovary cells and Kv4.2 and Kv4.3 currents in Xenopus oocytes by arachidonic acid at physiological concentrations. This concentration-dependent effect of arachidonic acid resembled that observed in cerebellar granule neurons and was fully reversible. Other fatty acids, including a nonhydrolyzable inhibitor of both lipooxygenase and cyclooxygenase, 5,8,11,14-eicosatetraynoic acid (ETYA), also mimicked arachidonic acid in modulating Kv4.3 and Kv4.3/KChIP1 currents. Compared with another transient potassium current formed by Kv1.1/Kvβ1, Kv4.3/KChIP1 current was much more sensitive to arachidonic acid. Association between KChIP1 and Kv4.2 or Kv4.3 was not altered in the presence of 10 μm ETYA as measured by immunoprecipitation and association-dependent growth in yeast. Our data suggest that the KChIP proteins represent a molecular entity for the observed difference between arachidonic acid effects on A-current kinetics in heterologous cells and in native cells and are consistent with the notion that KChIP proteins modulate the subthreshold A-current in neurons.