%0 Journal Article %A Xin Fang %A Laiche Djouhri %A Joel A. Black %A Sulayman D. Dib-Hajj %A Stephen G. Waxman %A Sally N. Lawson %T The Presence and Role of the Tetrodotoxin-Resistant Sodium Channel Nav1.9 (NaN) in Nociceptive Primary Afferent Neurons %D 2002 %R 10.1523/JNEUROSCI.22-17-07425.2002 %J The Journal of Neuroscience %P 7425-7433 %V 22 %N 17 %X This is the first examination of sensory receptive properties and associated electrophysiological properties in vivo of dorsal root ganglion (DRG) neurons that express the TTX-resistant sodium channel Nav1.9 (NaN). Intracellular recordings in lumbar DRGs in Wistar rats enabled units with dorsal root C-, Aδ-, or Aα/β-fibers to be classified as nociceptive, low-threshold mechanoreceptive (LTM), or unresponsive. Intracellular dye injection enabled subsequent immunocytochemistry for Nav1.9-like immunoreactivity (Nav1.9-LI).Nav1.9-LI was expressed selectively in nociceptive-type (C- and A-fiber nociceptive and C-unresponsive) units. Of the nociceptive units, 64, 54, and 31% of C-, Aδ-, and Aα/β-fiber units, respectively, were positive for Nav1.9-LI. C-unresponsive units were included in the nociceptive-type group on the basis of their nociceptor-like membrane properties; 91% were positive. Nav1.9-LI was undetectable in Aδ- or Aα/β-fiber LTM units and in one C-LTM unit. Nav1.9-LI intensity was correlated negatively with soma size and conduction velocity in nociceptive units and with conduction velocity in C-fiber units. There was a positive correlation with action potential rise time in nociceptive-type units with membrane potentials equal to or more negative than –50 mV. The data provide direct evidence that Nav1.9 is expressed selectively in (but not in all) C- and A-fiber nociceptive-type units and suggest that Nav1.9 contributes to membrane properties that are typical of nociceptive neurons. %U https://www.jneurosci.org/content/jneuro/22/17/7425.full.pdf