@article {Bernstein1, author = {Barbara W. Bernstein and James R. Bamburg}, title = {Actin-ATP Hydrolysis Is a Major Energy Drain for Neurons}, volume = {23}, number = {1}, pages = {1--6}, year = {2003}, doi = {10.1523/JNEUROSCI.23-01-00002.2003}, publisher = {Society for Neuroscience}, abstract = {In cultured chick ciliary neurons, when ATP synthesis is inhibited, ATP depletion is reduced \~{}50\% by slowing actin filament turnover with jasplakinolide or latrunculin A. Jasplakinolide inhibits actin disassembly, and latrunculin A prevents actin assembly by sequestering actin monomers. Cytochalasin D, which allows assembly{\textendash}disassembly, but only at pointed ends, is less effective in conserving ATP. Ouabain, an Na+{\textendash}K+-ATPase inhibitor, and jasplakinolide both prevent \~{}50\% of the ATP loss. When applied together, they completely prevent ATP loss over a period of 20 min, suggesting that filament stabilization reduces ATP consumption by decreasing actin-ATP hydrolysis directly rather than indirectly by modulating the activity of Na+{\textendash}K+-ATPase, a major energy consumer.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/23/1/1.2}, eprint = {https://www.jneurosci.org/content}, journal = {Journal of Neuroscience} }