RT Journal Article SR Electronic T1 Possible Involvement of P2Y2 Metabotropic Receptors in ATP-Induced Transient Receptor Potential Vanilloid Receptor 1-Mediated Thermal Hypersensitivity JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 6058 OP 6062 DO 10.1523/JNEUROSCI.23-14-06058.2003 VO 23 IS 14 A1 Moriyama, Tomoko A1 Iida, Tohko A1 Kobayashi, Kimiko A1 Higashi, Tomohiro A1 Fukuoka, Tetsuo A1 Tsumura, Hideki A1 Leon, Catherine A1 Suzuki, Noboru A1 Inoue, Kazuhide A1 Gachet, Christian A1 Noguchi, Koichi A1 Tominaga, Makoto YR 2003 UL http://www.jneurosci.org/content/23/14/6058.abstract AB The capsaicin receptor transient receptor potential V1 (TRPV1; also known as vanilloid receptor 1) is a sensory neuron-specific ion channel that serves as a polymodal detector of pain-producing chemical and physical stimuli. It has been reported that extracellular ATP potentiates the TRPV1 currents evoked by capsaicin or protons and reduces the temperature threshold for its activation through metabotropic P2Y receptors in a PKC-dependent pathway, suggesting that TRPV1 activation could trigger the sensation of pain at normal body temperature in the presence of ATP. Here, we show that ATP-induced thermal hyperalgesia was abolished in mice lacking TRPV1, suggesting the functional interaction between ATP and TRPV1 at a behavioral level. However, thermal hyperalgesia was preserved in P2Y1 receptor-deficient mice. Patch-clamp analyses using mouse dorsal root ganglion neurons indicated the involvement of P2Y2 rather than P2Y1 receptors. Coexpression of TRPV1 mRNA with P2Y2 mRNA, but not P2Y1 mRNA, was determined in the rat lumbar DRG using in situ hybridization histochemistry. These data indicate the importance of metabotropic P2Y2 receptors in nociception through TRPV1.