RT Journal Article
SR Electronic
T1 Molecular-Imaging Probe 2-(1-{6-[(2-Fluoroethyl)(Methyl)  Amino]-2-Naphthyl}Ethylidene) Malononitrile Labels Prion Plaques <em>In  Vitro</em>
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 8029
OP 8033
DO 10.1523/JNEUROSCI.23-22-08029.2003
VO 23
IS 22
A1 Mara Bresjanac
A1 Lojze M. Smid
A1 Tomaz D. Vovko
A1 Andrej Petrič
A1 Jorge R. Barrio
A1 Mara Popovic
YR 2003
UL http://www.jneurosci.org/content/23/22/8029.abstract
AB The study aimed to evaluate the fluorescent molecular-imaging probe 2-(1-{6-[(2-fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene)malononitrile (FDDNP) for its ability to selectively and reproducibly label prion plaques in fixed, paraffin-embedded cerebellar sections from patients of confirmed Gerstmann-Sträussler-Scheinker disease, sporadic Creutzfeldt-Jacob disease (CJD) with kuru plaques, and variant CJD (vCJD). FDDNP is a highly hydrophobic, viscosity-sensitive, solvent-sensitive, fluorescent substance, whose radiofluorinated analog [18F]FDDNP has recently been successfully used to label senile plaques and neurofibrillary tangles in the living brain of Alzheimer's disease patients with positron emission tomography. Our results show that FDDNP reliably identifies all prion plaques, including small cluster-plaques in vCJD. This finding may open new in vivo diagnostic possibilities for vCJD.