TY - JOUR T1 - Optical Quantal Analysis Indicates That Long-Term Potentiation at Single Hippocampal Mossy Fiber Synapses Is Expressed through Increased Release Probability, Recruitment of New Release Sites, and Activation of Silent Synapses JF - The Journal of Neuroscience JO - J. Neurosci. SP - 3618 LP - 3626 DO - 10.1523/JNEUROSCI.3567-03.2004 VL - 24 IS - 14 AU - Christopher A. Reid AU - Don B. Dixon AU - Michiko Takahashi AU - Tim V. P. Bliss AU - Alan Fine Y1 - 2004/04/07 UR - http://www.jneurosci.org/content/24/14/3618.abstract N2 - It is generally believed that long-term potentiation (LTP) at hippocampal mossy fiber synapses between dentate granule and CA3 pyramidal cells is expressed through presynaptic mechanisms leading to an increase in quantal content. The source of this increase has remained undefined but could include enhanced probability of transmitter release at existing functional release sites or increases in the number of active release sites. We performed optical quantal analyses of transmission at individual mossy fiber synapses in cultured hippocampal slices, using confocal microscopy and intracellular fluorescent Ca2+ indicators. Our results indicate that LTP is expressed at functional synapses by both increased probability of transmitter release and recruitment of new release sites, including the activation of previously silent synapses here visualized for the first time. ER -