RT Journal Article
SR Electronic
T1 Nociceptors Lacking TRPV1 and TRPV2 Have Normal Heat Responses
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 6410
OP 6415
DO 10.1523/JNEUROSCI.1421-04.2004
VO 24
IS 28
A1 C. Jeffery Woodbury
A1 Melissa Zwick
A1 Shuying Wang
A1 Jeffrey J. Lawson
A1 Michael J. Caterina
A1 Martin Koltzenburg
A1 Kathryn M. Albers
A1 H. Richard Koerber
A1 Brian M. Davis
YR 2004
UL http://www.jneurosci.org/content/24/28/6410.abstract
AB Vanilloid receptor 1 (TRPV1) has been proposed to be the principal heat-responsive channel for nociceptive neurons. The skin of both rat and mouse receives major projections from primary sensory afferents that bind the plant lectin isolectin B4 (IB4). The majority of IB4-positive neurons are known to be heat-responsive nociceptors. Previous studies suggested that, unlike rat, mouse IB4-positive cutaneous afferents did not express TRPV1 immunoreactivity. Here, multiple antisera were used to confirm that mouse and rat have different distributions of TRPV1 and that TRPV1 immunoreactivity is absent in heat-sensitive nociceptors. Intracellular recording in TRPV1-/- mice was then used to confirm that TRPV1 was not required for detecting noxious heat. TRPV1-/- mice had more heat-sensitive neurons, and these neurons had normal temperature thresholds and response properties. Moreover, in TRPV1-/- mice, 82% of heat-responsive neurons did not express immunoreactivity for TRPV2, another putative noxious heat channel.