RT Journal Article
SR Electronic
T1 Two Interdependent TRPV Channel Subunits, Inactive and Nanchung, Mediate Hearing in <em>Drosophila</em>
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 9059
OP 9066
DO 10.1523/JNEUROSCI.1645-04.2004
VO 24
IS 41
A1 Gong, Zhefeng
A1 Son, Wonseok
A1 Doo Chung, Yun
A1 Kim, Janghwan
A1 Shin, Dong Wook
A1 McClung, Colleen A.
A1 Lee, Yong
A1 Lee, Hye Won
A1 Chang, Deok-Jin
A1 Kaang, Bong-Kiun
A1 Cho, Hawon
A1 Oh, Uhtaek
A1 Hirsh, Jay
A1 Kernan, Maurice J.
A1 Kim, Changsoo
YR 2004
UL http://www.jneurosci.org/content/24/41/9059.abstract
AB Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons in Johnston's organ, the antennal chordotonal organ. We previously found that a Drosophila protein in the vanilloid receptor subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to the chordotonal cilia and required to generate sound-evoked potentials (Kim et al., 2003). Here, we show that the only other Drosophila TRPV protein is mutated in the behavioral mutant inactive (iav). The IAV protein forms a hypotonically activated channel when expressed in cultured cells; in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing. IAV and NAN are each undetectable in cilia of mutants lacking the other protein, indicating that they both contribute to a heteromultimeric transduction channel in vivo. A functional green fluorescence protein-IAV fusion protein shows that the channel is restricted to the proximal cilium, constraining models for channel activation.