PT  - JOURNAL ARTICLE
AU  - Gary Aston-Jones
AU  - Yan Zhu
AU  - J. Patrick Card
TI  - Numerous GABAergic Afferents to Locus Ceruleus in the Pericerulear Dendritic Zone: Possible Interneuronal Pool
AID  - 10.1523/JNEUROSCI.5339-03.2004
DP  - 2004 Mar 03
TA  - The Journal of Neuroscience
PG  - 2313--2321
VI  - 24
IP  - 9
4099  - http://www.jneurosci.org/content/24/9/2313.short
4100  - http://www.jneurosci.org/content/24/9/2313.full
SO  - J. Neurosci.2004 Mar 03; 24
AB  - Most nuclei in the CNS are composed of principal neurons that project to other areas and interneurons that serve to integrate information among afferents. The noradrenergic brain nucleus locus ceruleus (LC) has appeared to be an exception to this general rule, because the LC is composed almost entirely of noradrenergic principal neurons. Here, we report that numerous small neurons in the peri-LC region become retrogradely labeled after focal injections of wheat germ agglutinin-apo (inactivated) horseradish peroxidase conjugated to colloidal gold, or pseudorabies virus (PRV), into the nuclear core of the rat LC. A substantial number of these neurons were routinely found within the dendritic field of the LC, in the area surrounding the compact cell-dense region classically defined as LC. Double labeling revealed that a large percentage of these cells stained for GABA. Ultrastructural analyses revealed axodendritic and axosomatic contacts between PRV-labeled afferents and LC neurons labeled with tyrosine hydroxylase immunohistochemistry. In addition, PRV-labeled neurons or axons were immunopositive for GABA in ultrastructural localizations. Analysis of the synaptology of immunopositive profiles demonstrated that these LC afferents in the peri-LC region receive several non-LC synaptic inputs. These results indicate that a population of small GABAergic neurons in the peri-LC dendritic zone may provide interneuronal integration for LC noradrenergic neurons.