TY - JOUR T1 - Spinal G-Protein-Gated Potassium Channels Contribute in a Dose-Dependent Manner to the Analgesic Effect of μ- and δ- But Not κ-Opioids JF - The Journal of Neuroscience JO - J. Neurosci. SP - 3551 LP - 3559 DO - 10.1523/JNEUROSCI.4899-04.2005 VL - 25 IS - 14 AU - Cheryl L. Marker AU - Rafael Luján AU - Horace H. Loh AU - Kevin Wickman Y1 - 2005/04/06 UR - http://www.jneurosci.org/content/25/14/3551.abstract N2 - Opioids can evoke analgesia by inhibiting neuronal targets in either the brain or spinal cord, and multiple presynaptic and postsynaptic inhibitory mechanisms have been implicated. The relative significance of presynaptic and postsynaptic inhibition to opioid analgesia is essentially unknown, as are the identities and relevant locations of effectors mediating opioid actions. Here, we examined the distribution of G-protein-gated potassium (GIRK) channels in the mouse spinal cord and measured their contribution to the analgesia evoked by spinal administration of opioid receptor-selective agonists. We found that the GIRK channel subunits GIRK1 and GIRK2 were concentrated in the outer layer of the substantia gelatinosa of the dorsal horn. GIRK1 and GIRK2 were found almost exclusively in postsynaptic membranes of putative excitatory synapses, and a significant degree of overlap with the μ-opioid receptor was observed. Although most GIRK subunit labeling was perisynaptic or extrasynaptic, GIRK2 was found occasionally within the synaptic specialization. Genetic ablation or pharmacologic inhibition of spinal GIRK channels selectively blunted the analgesic effect of high but not lower doses of the μ-opioid receptor-selective agonist [d-Ala(2),N-Me-Phe(4),Gly(5)-ol]-enkephalin. Dose-dependent contributions of GIRK channels to the analgesic effects of the δ-opioid receptor-selective agonists Tyr-d-Ala-Phe-Glu-Val-Val-Gly amide and [d-Pen(2,5)]-enkephalin were also observed. In contrast, the analgesic effect of the κ agonist (trans)-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)-cyclohexyl] benzeneacetamide methanesulfonate hydrate was preserved despite the absence of GIRK channels. We conclude that the activation of postsynaptic GIRK1 and/or GIRK2-containing channels in the spinal cord dorsal horn represents a powerful, albeit relatively insensitive, means by which intrathecal μ- and δ-selective opioid agonists evoke analgesia. ER -