RT Journal Article
SR Electronic
T1 Local Protein Synthesis Mediates a Rapid Increase in Dendritic Elongation Factor 1A after Induction of Late Long-Term Potentiation
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 5833
OP 5843
DO 10.1523/JNEUROSCI.0599-05.2005
VO 25
IS 24
A1 Tsokas, Panayiotis
A1 Grace, Elizabeth A.
A1 Chan, PokMan
A1 Ma, Tao
A1 Sealfon, Stuart C.
A1 Iyengar, Ravi
A1 Landau, Emmanuel M.
A1 Blitzer, Robert D.
YR 2005
UL http://www.jneurosci.org/content/25/24/5833.abstract
AB The maintenance of long-term potentiation (LTP) requires a brief period of accelerated protein synthesis soon after synaptic stimulation, suggesting that an early phase of enhanced translation contributes to stable LTP. The mechanism regulating protein synthesis and the location and identities of mRNAs translated are not well understood. Here, we show in acute brain slices that the induction of protein synthesis-dependent hippocampal LTP increases the expression of elongation factor 1A (eEF1A), the mRNA of which contains a 5′ terminal oligopyrimidine tract. This effect is blocked by rapamycin, indicating that the increase in EF1A expression is mediated by the mammalian target of rapamycin (mTOR) pathway. We find that mRNA for eEF1A is present in pyramidal cell dendrites and that the LTP-associated increase in eEF1A expression was intact in dendrites that had been severed from their cell bodies before stimulation. eEF1A levels increased within 5 min after stimulation in a translation-dependent manner, and this effect remained stable for 3 h. These results suggest a mechanism whereby synaptic stimulation, by signaling through the mTOR pathway, produces an increase in dendritic translational capacity that contributes to LTP maintenance.