TY - JOUR T1 - Double Patch Clamp Reveals That Transient Fusion (Kiss-and-Run) Is a Major Mechanism of Secretion in Calf Adrenal Chromaffin Cells: High Calcium Shifts the Mechanism from Kiss-and-Run to Complete Fusion JF - The Journal of Neuroscience JO - J. Neurosci. SP - 3030 LP - 3036 DO - 10.1523/JNEUROSCI.5275-05.2006 VL - 26 IS - 11 AU - Abdeladim Elhamdani AU - Fouad Azizi AU - Cristina R. Artalejo Y1 - 2006/03/15 UR - http://www.jneurosci.org/content/26/11/3030.abstract N2 - Transient fusion (“kiss-and-run”) is accepted as a mode of transmitter release both in central neurons and neuroendocrine cells, but the prevalence of this mechanism compared with full fusion is still in doubt. Using a novel double patch-clamp method (whole cell/cell attached), permitting the recording of unitary capacitance events while stimulating under a variety of conditions including action potentials, we show that transient fusion is the predominant (>90%) mode of secretion in calf adrenal chromaffin cells. Raising intracellular Ca2+ concentration ([Ca]i) from 10 to 200 μm increases the incidence of full fusion events at the expense of transient fusion. Blocking rapid endocytosis that normally terminates transient fusion events also promotes full fusion events. Thus, [Ca]i controls the transition between transient and full fusion, each of which is coupled to different modes of endocytosis. ER -