@article {Dimou5591, author = {Leda Dimou and Lisa Schnell and Laura Montani and Carri Duncan and Marjo Simonen and Regula Schneider and Thomas Liebscher and Miriam Gullo and Martin E. Schwab}, title = {Nogo-A-Deficient Mice Reveal Strain-Dependent Differences in Axonal Regeneration}, volume = {26}, number = {21}, pages = {5591--5603}, year = {2006}, doi = {10.1523/JNEUROSCI.1103-06.2006}, publisher = {Society for Neuroscience}, abstract = {Nogo-A, a membrane protein enriched in myelin of the adult CNS, inhibits neurite growth and regeneration; neutralizing antibodies or receptor blockers enhance regeneration and plasticity in the injured adult CNS and lead to improved functional outcome. Here we show that Nogo-A-specific knock-outs in backcrossed 129X1/SvJ and C57BL/6 mice display enhanced regeneration of the corticospinal tract after injury. Surprisingly, 129X1/SvJ Nogo-A knock-out mice had two to four times more regenerating fibers than C57BL/6 Nogo-A knock-out mice. Wild-type newborn 129X1/SvJ dorsal root ganglia in vitro grew a much higher number of processes in 3 d than C57BL/6 ganglia, confirming the stronger endogenous neurite growth potential of the 129X1/SvJ strain. cDNA microarrays of the intact and lesioned spinal cord of wild-type as well as Nogo-A knock-out animals showed a number of genes to be differentially expressed in the two mouse strains; many of them belong to functional categories associated with neurite growth, synapse formation, and inflammation/immune responses. These results show that neurite regeneration in vivo, under the permissive condition of Nogo-A deletion, and neurite outgrowth in vitro differ significantly in two widely used mouse strains and that Nogo-A is an important endogenous inhibitor of axonal regeneration in the adult spinal cord.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/26/21/5591}, eprint = {https://www.jneurosci.org/content/26/21/5591.full.pdf}, journal = {Journal of Neuroscience} }