PT  - JOURNAL ARTICLE
AU  - Hull, Court
AU  - Li, Geng-Lin
AU  - von Gersdorff, Henrique
TI  - GABA Transporters Regulate a Standing GABA<sub>C</sub> Receptor-Mediated Current at a Retinal Presynaptic Terminal
AID  - 10.1523/JNEUROSCI.1386-06.2006
DP  - 2006 Jun 28
TA  - The Journal of Neuroscience
PG  - 6979--6984
VI  - 26
IP  - 26
4099  - http://www.jneurosci.org/content/26/26/6979.short
4100  - http://www.jneurosci.org/content/26/26/6979.full
SO  - J. Neurosci.2006 Jun 28; 26
AB  - At the axon terminal of goldfish retinal bipolar cells, GABAC receptors have been shown to mediate inhibitory reciprocal synaptic currents. Here, we demonstrate a novel standing GABAergic current mediated exclusively by GABAC receptors. Selective inhibition of GAT-1 GABA transporters on amacrine cells increases this tonic current and reveals a specific functional coupling between GAT-1 transporters and GABAC receptors. We propose that this GABAC receptor-mediated standing current serves to regulate synaptic gain by shunting depolarizing potentials that can produce Ca2+-dependent action potentials at the bipolar cell terminal. Furthermore, we find that the amount of GABAC receptor-mediated reciprocal feedback between bipolar cell terminals and amacrine cells is greatly increased when GAT-1 transporters are specifically blocked by NO-711 (1-[2-[[(diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride). The involvement of GAT-1 transporters in regulating this standing (or tonic) GABAC current implicates them in a novel role as major determinants of presynaptic excitability.