RT Journal Article SR Electronic T1 GABA Transporters Regulate a Standing GABAC Receptor-Mediated Current at a Retinal Presynaptic Terminal JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 6979 OP 6984 DO 10.1523/JNEUROSCI.1386-06.2006 VO 26 IS 26 A1 Hull, Court A1 Li, Geng-Lin A1 von Gersdorff, Henrique YR 2006 UL http://www.jneurosci.org/content/26/26/6979.abstract AB At the axon terminal of goldfish retinal bipolar cells, GABAC receptors have been shown to mediate inhibitory reciprocal synaptic currents. Here, we demonstrate a novel standing GABAergic current mediated exclusively by GABAC receptors. Selective inhibition of GAT-1 GABA transporters on amacrine cells increases this tonic current and reveals a specific functional coupling between GAT-1 transporters and GABAC receptors. We propose that this GABAC receptor-mediated standing current serves to regulate synaptic gain by shunting depolarizing potentials that can produce Ca2+-dependent action potentials at the bipolar cell terminal. Furthermore, we find that the amount of GABAC receptor-mediated reciprocal feedback between bipolar cell terminals and amacrine cells is greatly increased when GAT-1 transporters are specifically blocked by NO-711 (1-[2-[[(diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride). The involvement of GAT-1 transporters in regulating this standing (or tonic) GABAC current implicates them in a novel role as major determinants of presynaptic excitability.