TY - JOUR T1 - Bidirectional Activity-Dependent Regulation of Neuronal Ion Channel Phosphorylation JF - The Journal of Neuroscience JO - J. Neurosci. SP - 13505 LP - 13514 DO - 10.1523/JNEUROSCI.3970-06.2006 VL - 26 IS - 52 AU - Hiroaki Misonou AU - Milena Menegola AU - Durga P. Mohapatra AU - Lauren K. Guy AU - Kang-Sik Park AU - James S. Trimmer Y1 - 2006/12/27 UR - http://www.jneurosci.org/content/26/52/13505.abstract N2 - Activity-dependent dephosphorylation of neuronal Kv2.1 channels yields hyperpolarizing shifts in their voltage-dependent activation and homoeostatic suppression of neuronal excitability. We recently identified 16 phosphorylation sites that modulate Kv2.1 function. Here, we show that in mammalian neurons, compared with other regulated sites, such as serine (S)563, phosphorylation at S603 is supersensitive to calcineurin-mediated dephosphorylation in response to kainate-induced seizures in vivo, and brief glutamate stimulation of cultured hippocampal neurons. In vitro calcineurin digestion shows that supersensitivity of S603 dephosphorylation is an inherent property of Kv2.1. Conversely, suppression of neuronal activity by anesthetic in vivo causes hyperphosphorylation at S603 but not S563. Distinct regulation of individual phosphorylation sites allows for graded and bidirectional homeostatic regulation of Kv2.1 function. S603 phosphorylation represents a sensitive bidirectional biosensor of neuronal activity. ER -