RT Journal Article
SR Electronic
T1 Multiple Modes of Synaptic Excitation of Olfactory Bulb Granule Cells
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 5621
OP 5632
DO 10.1523/JNEUROSCI.4630-06.2007
VO 27
IS 21
A1 Ramani Balu
A1 R. Todd Pressler
A1 Ben W. Strowbridge
YR 2007
UL http://www.jneurosci.org/content/27/21/5621.abstract
AB Inhibition generated by granule cells, the most common GABAergic cell type in the olfactory bulb, plays a critical role in shaping the output of the olfactory bulb. However, relatively little is known about the synaptic mechanisms responsible for activating these interneurons in addition to the specialized dendrodendritic synapses located on distal dendrites. Using two-photon guided minimal stimulation in acute rat brain slices, we found that distal and proximal excitatory synapses onto granule cells are functionally distinct. Proximal synapses arise from piriform cortical neurons and facilitate with paired-pulse stimulation, whereas distal dendrodendritic synapses generate EPSCs with slower kinetics that depress with paired stimulation. Proximal cortical feedback inputs can relieve the tonic Mg block of NMDA receptors (NMDARs) at distal synapses and gate dendrodendritic inhibition onto mitral cells. Most excitatory synapses we examined onto granule cells activated both NMDARs and AMPA receptors, whereas a subpopulation appeared to be NMDAR silent. The convergence of two types of excitatory inputs onto GABAergic granule cells provides a novel mechanism for regulating the degree of interglomerular processing of sensory input in the olfactory bulb through piriform cortex/olfactory bulb synaptic interactions.