TY - JOUR T1 - The Transduction Channel TRPM5 Is Gated by Intracellular Calcium in Taste Cells JF - The Journal of Neuroscience JO - J. Neurosci. SP - 5777 LP - 5786 DO - 10.1523/JNEUROSCI.4973-06.2007 VL - 27 IS - 21 AU - Zheng Zhang AU - Zhen Zhao AU - Robert Margolskee AU - Emily Liman Y1 - 2007/05/23 UR - http://www.jneurosci.org/content/27/21/5777.abstract N2 - Bitter, sweet, and umami tastants are detected by G-protein-coupled receptors that signal through a common second-messenger cascade involving gustducin, phospholipase C β2, and the transient receptor potential M5 (TRPM5) ion channel. The mechanism by which phosphoinositide signaling activates TRPM5 has been studied in heterologous cell types with contradictory results. To resolve this issue and understand the role of TRPM5 in taste signaling, we took advantage of mice in which the TRPM5 promoter drives expression of green fluorescent protein and mice that carry a targeted deletion of the TRPM5 gene to unequivocally identify TRPM5-dependent currents in taste receptor cells. Our results show that brief elevation of intracellular inositol trisphosphate or Ca2+ is sufficient to gate TRPM5-dependent currents in intact taste cells, but only intracellular Ca2+ is able to activate TRPM5-dependent currents in excised patches. Detailed study in excised patches showed that TRPM5 forms a nonselective cation channel that is half-activated by 8 μm Ca2+ and that desensitizes in response to prolonged exposure to intracellular Ca2+. In addition to channels encoded by the TRPM5 gene, we found that taste cells have a second type of Ca2+-activated nonselective cation channel that is less sensitive to intracellular Ca2+. These data constrain proposed models for taste transduction and suggest a link between receptor signaling and membrane potential in taste cells. ER -