TY - JOUR T1 - Protracted Synaptogenesis after Activity-Dependent Spinogenesis in Hippocampal Neurons JF - The Journal of Neuroscience JO - J. Neurosci. SP - 8149 LP - 8156 DO - 10.1523/JNEUROSCI.0511-07.2007 VL - 27 IS - 30 AU - U. Valentin Nägerl AU - German Köstinger AU - John C. Anderson AU - Kevan A. C. Martin AU - Tobias Bonhoeffer Y1 - 2007/07/25 UR - http://www.jneurosci.org/content/27/30/8149.abstract N2 - Activity-dependent morphological plasticity of neurons is central to understanding how the synaptic network of the CNS becomes reconfigured in response to experience. In recent years, several studies have shown that synaptic activation that leads to the induction of long-term potentiation also drives the growth of new dendritic spines, raising the possibility that new synapses are made. We examine this directly by correlating time-lapse two-photon microscopy of newly formed spines on CA1 pyramidal neurons in organotypic hippocampal slices with electron microscopy. Our results show that, whereas spines that are only a few hours old rarely form synapses, older spines, ranging from 15 to 19 h, consistently have ultrastructural hallmarks typical of synapses. This is in agreement with a recent in vivo study that showed that, after a few days, new spines consistently form functional synapses. In addition, our study provides a much more detailed understanding of the first few hours after activity-dependent spinogenesis. Within tens of minutes, physical contacts are formed with existing presynaptic boutons, which slowly, over the course of many hours, mature into new synapses. ER -