RT Journal Article
SR Electronic
T1 Gβ5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 14199
OP 14204
DO 10.1523/JNEUROSCI.4934-07.2007
VO 27
IS 51
A1 Rao, Anjali
A1 Dallman, Rebecca
A1 Henderson, Scott
A1 Chen, Ching-Kang
YR 2007
UL http://www.jneurosci.org/content/27/51/14199.abstract
AB Gβ5 exists as two splice variants, Gβ5-S and Gβ5-L, which interact with and stabilize the R7 members of the regulators of G-protein signaling (RGSs): RGS6, RGS7, RGS9, and RGS11. Although the role of Gβ5-L and RGS9-1 is established in photoreceptors, the physiological functions of Gβ5-S and other R7 RGS proteins remain unclear. We found that the electroretinogram of Gβ5−/− mice lacks the b-wave component and that Gβ5-S and RGS11 colocalize with Goα at the tips of the ON-bipolar cell dendrites. Unexpectedly, we found a significant reduction in the number of synaptic triads in the outer plexiform layer (OPL) of the Gβ5−/− mice, which is evident at postnatal day 14. Transgenic expression of Gβ5-L in rods failed to rescue the b-wave or the OPL defects. These results indicate that Gβ5-S is indispensable for OPL integrity and normal light responses of the retina.