RT Journal Article
SR Electronic
T1 GABAergic Neurons Are Less Selective to Stimulus Orientation than Excitatory Neurons in Layer II/III of Visual Cortex, as Revealed by <em>In Vivo</em> Functional Ca<sup>2+</sup> Imaging in Transgenic Mice
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 2145
OP 2149
DO 10.1523/JNEUROSCI.4641-06.2007
VO 27
IS 8
A1 Sohya, Kazuhiro
A1 Kameyama, Katsuro
A1 Yanagawa, Yuchio
A1 Obata, Kunihiko
A1 Tsumoto, Tadaharu
YR 2007
UL http://www.jneurosci.org/content/27/8/2145.abstract
AB Most neurons in the visual cortex are selectively responsive to visual stimulation of a narrow range of orientations, and GABAergic neurons are considered to play a role in the formation of such orientation selectivity. This suggests that response properties of GABAergic neurons may be different from those of excitatory neurons. This view remains unproved, however. To address this issue, we applied in vivo two-photon functional Ca2+ imaging to transgenic mice, in which GABAergic neurons express enhanced green fluorescent protein. Astroglia were stained by an astrocyte-specific dye. The three types of cells, GABAergic neurons, excitatory neurons, and astrocytes, in layer II/III of the visual cortex were differentially identified by using different wavelengths of excitation light and a dichroic mirror for emitted fluorescence, and their responses to moving visual stimuli at different orientations were measured with changes in the intensity of fluorescence of a Ca2+-sensitive dye. We found that almost all GABAergic neurons have orientation-insensitive responses, whereas most of excitatory neurons have orientation-selective responses.